Coding

Part:BBa_K5181001

Designed by: Mayukh Mitra   Group: iGEM24_IISER-TVM   (2024-08-08)


P450_truncated: Santalum album

This part is a modified form of the Part BBa_K5181000 (P450_native). We aimed to make a Eukaryotic plant transmembrane P450 express itself in a prokaryotic system (Pseudomonas putida). Thus, we truncated the transmembrane domain by removing 64 nucleotides from the N-terminal End, excluding the start codon.

The Alphafoldpredicted structure of the protein is:

The transmembrane tendency of wild type and truncated P450


The extreme N-terminal and C-terminal residues show low transmembrane(below 0) tendencies and thus we can presume the protein will not get embedded to the membrane, Thus it behaves like P.putida own P450

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1162
    Illegal PstI site found at 604
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1162
    Illegal PstI site found at 604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1162
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1162
    Illegal PstI site found at 604
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1162
    Illegal PstI site found at 604
  • 1000
    COMPATIBLE WITH RFC[1000]


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